Table 2.
Primers and probes used in this study
| Targeted microorganisms | Targeted gene | Primers and probea | Sequences 5′-3′ | Annealing temperature (°C) | References |
|---|---|---|---|---|---|
| qPCR | |||||
| Anaplasmataceae | 23S rRNA | TtAna-F TtAna-R TtAna-Sa |
TGACAGCGTACCTTTTGCAT GTAACAGGTTCGGTCCTCCA FAM-CTTGGTTTCGGGTCTAATCC-TAMRA |
60 | [28, 29] |
| Conventional PCR | |||||
| Anaplasmataceae | 23S rRNA | Ana23S-212f Ana23S-753r |
ATAAGCTGCGGGGAGTTGTC TGCAAAAGGTACGCTGTCAC |
55 | [28, 29] |
| Anaplasma spp. | rpoB | Ana-rpoBF Ana-rpoBR |
GCTGTTCCTAGGCTYTCTTACGCGA AATCRAGCCAVGAGCCCCTRTAWGG |
55 | [27] |
| Ehrlichia spp. | groEL | Ehr-groEL-F Ehr-groEL-R |
GTTGAAAARACTGATGGTATGCA ACACGRTCTTTACGYTCYTTAAC |
50 | [27] |
| Ticks | 12S rRNA | T1B T2A |
AAACTAGGATTAGATACCCT AATGAGAGCGACGGGCGATGT |
51 | [26] |
aProbe