Table 1.
Gene | Accession number1 | Primer sequence (5'→3') | Product (bp) |
β-actin | NM_173979.3 | F: CAGAAGGACTCGTACGTGGG R: TTGGCCTTGGGGTTCAGGG | 199 |
TNF-α | NM_173966.3 | F: CTTCATTGCCAGGTTTCTG R: CAGGTGTTGGATGCAGCTCT | 141 |
IL-1β | NM_174093.1 | F: ATGACTTCTGCTTTCCCTACCC R: GCTGCTTTCACACTCATCATTC | 179 |
IL-6 | NM_173923.2 | F: GCAFFTATTTGTGAAGAGAGCTG R: CACAGAACATGAGGCACTGAA | 148 |
IL-8 | NM_173925.2 | F: ACGGGCTTTACCTCATCTACTC R: GCTCTTGATGGCAGACAGG | 140 |
Primers were designed from the sequences published in the GenBank database under the indicated accession numbers. F: forward primer; R: reverse primer