Figure 2. ITK is required for parasitic/viral infection-induced Tr1 cell development in vivo.

(a,b) ITK is required for Tr1 cell development during parasitic infection: WT and Itk^−/− mice were infected with 500 L3 N. brasiliensis (N.B.) and lungs analyzed 7 days post infection (d.p.i.). (a) Representative FACS plots showing IL-10^GFP and Foxp3^RFP expression by pulmonary CD4⁺ T cells and summary of IL-10⁺Foxp3⁻ T cell percentage, number and IL-10 expression levels (WT average level set as 1). (b) Representative FACS plots showing the LAG3 and CD49b expression by IL-10^GFP+ Foxp3^RFP− CD4⁺ T cells, summary of LAG3⁺CD49b⁺ IL-10⁺Foxp3⁻CD4⁺ Tr1 cell percentage and number from samples shown in a. ^§P≤0.05, ^§§P≤0.01, ^§§§P≤0.001, compared with PBS-treated group; *P≤0.05, **P≤0.01, ***P≤0.001, comparing groups connected, by non-parametric Mann-Whitney test. (c,d) ITK is required for Tr1 cell development during viral infection: WT and Itk^−/− mice were infected with 10⁴ PFU Influenza A/WSN/1933 (WSN) and lungs analyzed 3 and 7 d.p.i. (c) Representative FACS plots showing IL-10^GFP and Foxp3^RFP expression by pulmonary CD4⁺ T cells and summary of IL-10⁺Foxp3⁻ T cell percentage, number and IL-10 expression levels (WT average level set as 1). (d) Representative FACS plots showing the LAG3/CD49b expression by IL-10^GFP+ Foxp3^RFP− CD4⁺ T cells, summary of LAG3⁺CD49b⁺ IL-10⁺Foxp3⁻CD4⁺ Tr1 cell percentage and number from samples shown in c. P values on plots were calculated by two-way ANOVA. Data were pooled from three experiments, ‘n’ indicates number of replicates in each group/point. Data presented as mean±s.e.m.