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. 2017 Jun 21;8:15831. doi: 10.1038/ncomms15831

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Cartoon representation of the optoPlexin design (see main text). FP, fluorescent protein; Plexin_cyto, cytosolic domain of Plexin-B1. (b) TIRF images showing the plasma membrane recruitment of mCherry-optoPlexin in COS-7 cells after 1 min of whole cell or local illumination (440 nm and 50 ms pulse at 0.1Hz, unless otherwise stated). Scale bar, 10 μm. White circle, region of illumination. (c) Quantification of the recruitment levels of Cry2, Cry2(D393A), optoPlexin and optoPlexin(D393A) on whole cell or localized blue light illumination in COS-7 cells. n=12–20, means±s.e.m. (d) TIRF images showing the clustering of mCherry-optoPlexin after one minute of whole-cell illumination in COS-7 cells. Scale bar, 10 μm. NS, not significant, Student’s t-test.