Table I.
Primer sequences for reverse transcription-quantitative polymerase chain reaction analysis.
| Gene | Primer sequence (5′→3′) |
|---|---|
| Sp7 | F: GAGGCACAAAGAAGCCATACA |
| R: GGGAAAGGGTGGGTAGTCAT | |
| OCN | F: ACAAGTCCCACACAGCAACTC |
| R: CCAGGTCAGAGAGGCAGAAT | |
| Runx2 | F: CACCTCTGACTTCTGCCTCTG |
| R: GATGAAATGCCTGGGAACTG | |
| OPN | F: CTTGGCTTACGGACTGAGG |
| R: GCAACTGGGATGACCTTGAT | |
| Smad7 | F: TCGGAAGTCAAGAGGCTGTG |
| R: CTGGACAGTCTGCAGTTGGTT | |
| MAPK9 | F: AACTCGCTACTATCGGGCTC |
| R: TGGGAACAGGACTTTATGGAGG | |
| Smad5 | F: CCAGTGTTAGTGCCTCGTCA |
| R: GTGGAAGGAATCAGGAAACG | |
| Bambi | F: CATTGCTGGCGGACTGAT |
| R: TCCCTTCTTGGAGTGGTGTC | |
| Dusp2 | F: GGTGGTCCTGTGGAAATCTT |
| R: GAATGCTCTTGTAGCGGAAAA | |
| Jag1 | F: TGCTTGGTGACAGCCTTCTA |
| R: TGGGGTTTTTGATTTGGTTC | |
| GAPDH | F: CAGTGCCAGCCTCGTCTCAT |
| R: AGGGGCCATCCACAGTCTTC |
Bambi, BMP and activin membrane-bound inhibitor; Dusp2, dual-specificity phosphatase 2; F, forward; Jag1, jagged 1; MAPK9, mitogen-activated protein kinase 9; OCN, osteocalcin; OPN, osteopontin; R, reverse; Runx2, runt-related 2.