Figure 5.

Effect of pro-inflammatory cytokines and/or tensile strength on ALP activity in PDLCs. ALP activity was assessed in PDLCs treated for 12, 24 or 48 h. Data are expressed as the mean ± standard deviation (n=3). *P<0.05, **P<0.01, ***P<0.001. I(−)/T(−), untreated cells; I(−)/T(+), cells treated with tensile strength alone; I(+)/T(−), cells treated with IL-1β/TNF-α alone; I(+)/T(+), cells treated with tensile strength and IL-1β/TNF-α at each time point. PDLCs, periodontal ligament cells; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; NS, non-significant; ALP, alkaline phosphatase.