Figure 6.

Mineralization potential of PDLCs exposed to pro-inflammatory stimuli and/or tensile strength. PDLCs were subjected to treatment conditions described in detail in the text for 48 h prior to being cultured in osteogenic medium for 14 days. (A) Alizarin red S staining was performed for 30 min after this culture period. In addition to (B) qualitative assessment of images of the wells, the absorbance of the solubilised stain was measured at a wavelength of 405 nm. Data are expressed as the mean ± standard deviation (n=3). *P<0.05, ***P<0.001. IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; PDLCs, periodontal ligament cells; OD, optical density.