Figure 2.

DEC2 inhibits expression of apoptotic markers following cisplatin treatment. TE-11 cells were transfected for 24 h with pcDNA, (A) DEC2 pcDNA or (B) DEC1 pcDNA. Transfected cells were incubated with 20 µM cisplatin for an additional 24 h, and then lysed. Cell lysates were subjected to western blot analysis for protein expression of DEC1, DEC2, cleaved PARP, cleaved caspase-8, cleaved caspase-3, Bim isoforms, Bax, Bcl-2, Bcl-xL and β-actin. Data are presented as the mean ± standard deviation, from three independent experiments. DEC, differentiated embryonic chondrocyte expressed gene; pcDNA, empty vector control; DEC2 pcDNA, DEC2 overexpression vector; DEC1 pcDNA, DEC1 overexpression vector; PARP, poly ADP-ribose polymerase; Bim, Bcl-2-interacting mediator of cell death; EL, extra long; L, long; S, short; Bax, Bcl-2 associated protein X apoptosis regulator; Bcl-2, B-cell lymphoma 2 apoptosis regulator; BcL-xL, Bcl-2-like 1 protein extra large.