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. 2017 Jun 15;66(7):1890–1900. doi: 10.2337/db16-1170

Figure 6.

Figure 6

Kv2.1 and a role for the channel in granule recruitment. A: Knockdown of Kv2.1 protein in Ad-shKv2.1–infected INS 832/13 cells compared with control subjects (Ad-shScrambled; n = 4 experiments). B and C: Knockdown of Kv2.1 in INS 832/13 cells resulted in a decreased density of membrane-resident secretory granules marked with NPY-Venus and visualized by TIRF microscopy at both 1 and 16.7 mmol/L glucose. Representative images (B) and quantified data (C) are shown (n = 30, 30, 27, 30, 28, and 30 cells in 3 experiments). Syn1A, syntaxin 1A. Scale bar, 10 μm. **P < 0.01; ***P < 0.001 compared with 1 mmol/L glucose control or as indicated.