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. 2017 Apr 19;66(7):1964–1978. doi: 10.2337/db16-1588

Figure 1.

Figure 1

A: Generation of db/db hnRNP F–Tg mice. Genotyping of hnRNP F–HA transgene, WT, and mutated (MUT) LepR gene by specific PCR analysis. B: Real-time qPCR for hnRNP F mRNA levels in freshly isolated RPTs from db/m, db/m hnRNP F–Tg, db/db, and db/db hnRNP F–Tg mice. C: Immunohistochemical staining for hnRNP F expression in kidney sections (original magnification ×200). D: WB analysis of hnRNP F protein expression in RPT extracts of male db/m, db/m hnRNP F–Tg, db/db, and db/db hnRNP F–Tg mice. E: Colocalization of immunostaining of hnRNP F and AQP1 in male db/m and db/m hnRNP F–Tg mouse kidneys (original magnification ×200). Scale bars = 50 μm. G, glomerulus; P, proximal tubule. *P < 0.05; **P < 0.01. F: Longitudinal changes in mean SBP in male db/m (□), db/m hnRNP F–Tg (■), db/db (△), and db/db hnRNP F–Tg (▲) mice. Values are means ± SEM, n = 10 for each group. *P < 0.05 compared to db/m mice; #P < 0.05 compared to db/db mice.