Figure 6.

rs11203203 and rs80054410 regulate the expression of UBASH3A and IL2 in human primary CD4⁺ T cells. A and B: Human primary CD4⁺ T cells were negatively selected from PBMCs from healthy subjects and then stimulated with anti-CD3 plus anti-CD28 or with culture medium for 6 h. The relative mRNA levels of UBASH3A and IL2 are shown. Each data point represents one subject, and the data are pooled from six quantitative PCR experiments. In A, the best-fit line generated by linear regression is represented by a solid line, and the 95% CI bands of the best-fit line are indicated by dashed curves. Genotypes at rs11203203 and rs80054410 are shown. In B, the mean and SEM values are indicated by solid lines and error bars, respectively. Unpaired one-tailed Student t tests were performed to assess statistical significance.