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. 2017 May 17;16(1):167–173. doi: 10.3892/mmr.2017.6598

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — CD133 expression and self-renewal following Fluorescence Activated Cell Sorting of GSCs. (A) qPCR and (B) western blot analysis demonstrating markedly increased expression levels of CD133 in CD133(+) GSCs. Gray value indicates protein optical density as analyzed by ImageJ software. GAPDH served as an internal control. (C) qPCR analysis revealed an array of stemness- and epithelial-mesenchymal transition-associated genes were upregulated in CD133(+) GSCs compared with CD133(−) NSCs. (D) A sphere formation assay demonstrated increased sphere formation in CD133(+) GSCs compared with CD133(−) NSCs. Data are presented as the mean ± standard error. *P<0.05 and **P<0.01 vs. NSCs. GSCs, glioblastoma stem cells; NSCs, non-glioblastoma stem cells; Oct-4, octamer-binding transcription factor 4; Nanog, homeobox protein Nanog; TWIST-1, twist family BHLH transcription factor 1; ZEB, zinc finger E-box binding homeobox; N, neural; E, epithelial; qPCR, quantitative polymerase chain reaction; CD133, cluster of differentiation 133.