Table I.
Quantitative polymerase chain reaction primers.
| Gene | Sequence (5′-3′) |
|---|---|
| CD133 | F: GGCCCAGTACAACACTACCAA |
| R: ATTCCGCCTCCTAGCACTGAA | |
| Oct-4 | F: CTGGGTTGATCCTCGGACCT |
| R: CCATCGGAGTTGCTCTCCA | |
| Nestin | F: CAACAGCGACGGAGGTCTC |
| R: GCCTCTACGCTCTCTTCTTTGA | |
| C-Myc | F: TCCCTCCACTCGGAAGGAC |
| R: CTGGTGCATTTTCGGTTGTTG | |
| Nanog | F: AAGGTCCCGGTCAAGAAACAG |
| R: CTTCTGCGTCACACCATTGC | |
| Jagged1 | F: GCCGAGGTCCTATACGTTGC |
| R: CCGAGTGAGAAGCCTTTTCAA | |
| TWIST-1 | F: GTCCGCAGTCTTACGAGGAG |
| R: GCTTGAGGGTCTGAATCTTGCT | |
| Vimentin | F: AGTCCACTGAGTACCGGAGAC |
| R: CATTTCACGCATCTGGCGTTC | |
| ZEB-1 | F: TTACACCTTTGCATACAGAACCC |
| R: TTTACGATTACACCCAGACTGC | |
| ZEB-2 | F: GGAGACGAGTCCAGCTAGTGT |
| R: CCACTCCACCCTCCCTTATTTC | |
| N-cadherin | F: AGCCAACCTTAACTGAGGAGT |
| R: GGCAAGTTGATTGGAGGGATG | |
| E-cadherin | F: ATTTTTCCCTCGACACCCGAT |
| R: TCCCAGGCGTAGACCAAGA |
F, forward; R, reverse; Oct-4, octamer-binding transcription factor 4; Nanog, homeobox protein Nanog; TWIST-1, twist family BHLH transcription factor 1; ZEB, zinc finger E-box binding homeobox; N, neural; E, epithelial; CD, cluster of differentiation.