Figure 4.

Baclofen has no direct effect on SLO2.1 channels. A, Whole-cell currents of SLO2.1 channels expressed in Xenopus oocytes before (control) and after the addition of 10 μM baclofen. The currents were evoked by voltage pulses from –100 to 40 mV in 10-mV steps at a holding potential of –80 mV. B, Representative I-V plot of currents from A shows that baclofen has no direct effect on SLO2.1 currents. Plot in C summarizes the current measurements at 40 mV before (control) and after baclofen treatment. Current values were normalized by control. Mean value (black horizontal line) and SD error bar are shown. There is no significant change in current amplitude after baclofen treatment (n = 5, p > 0.05).