Figure 5.

Combined effect of Bor+DNR in primary T-ALL cells. Primary blasts from five patients with T-ALL were exposed to Bor (10 nM) and DNR (500 nM) for 24 h, and the (A) apoptotic cells were determined using Annexin V/PI staining. (B) ΔΨm was determined using rhodamine123/PI double staining with flow cytometry. (C) Indicated proteins were examined using western blot analysis. Data are expressed as the mean ± standard deviation. Each experiment was performed at least 3 times independently. Images are representative of at least three independent experiments. *P<0.05, compared with Bor or DNR treatment. Bor, bortezomib; DNR, daunorubincin; PI, propidium iodide.