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. 2017 May 16;16(1):109–118. doi: 10.3892/mmr.2017.6586

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effect of octreotide on HFD-induced hepatic steatosis in obese rats and on PA-induced hepatocyte lipid accumulation in HepG2 cells. (A) Appearance of rat livers in each experimental group. Hepatic steatosis was evaluated by (B) hematoxylin and eosin staining of the paraffin-embedded liver tissue sections (magnification, ×400), and by (C) Oil Red O staining of frozen liver tissue sections (magnification, ×400). (D) Lipid accumulation in HepG2 cells was assessed by Oil Red O staining (magnification, ×400). The IOD values of Oil Red O staining in the (E) liver of rats and (F) in HepG2 cells. Data are presented as the mean ± standard deviation of three independent experiments. **P<0.01 vs. the control group; ^##P<0.01 vs. the HFD group or the PA group. HFD, high fat diet; PA, palmitate; IOD, integrated optical density.