Table I.
Primer sequences used for reverse transcription-quantitative polymerase chain reaction analysis.
| Gene (organism) | NCBI reference sequence | Forward sequence (5′-3′) | Reverse sequence (5′-3′) | Product size (bp) |
|---|---|---|---|---|
| β-actin (rat) | NM_031144 | CGAGTACAACCTTCTTGCAGC | CCTTCTGACCCATACCCACC | 209 |
| GS (rat) | NM_013089 | AAGAGTTTGTCCGAGGCTGTC | ACCAGAGAGGTTCGTAGTCACAC | 119 |
| β-actin (human) | NM_001101 | CACAGAGCCTCGCCTTT | GGTGCCAGATTTTCTCCAT | 318 |
| GS (human) | NM_002103 | GCCTTTCCAGAGCACTTCAC | CTCCTCGTCCTCATCGTAGC | 195 |
Primers were synthesized by TSINGKE Biological Technology, Co., Ltd. (Beijing, China). NCBI, National Centre for Biotechnology Information; GS, glycogen synthase.