Figure 4.

Effect of intravitreal injection of fasudil on axonal retraction by photoreceptors 2 hours after retinal detachment. (A) Representative images of retina labeled for SV2 (green) and nuclei (red). Control eye, detachment made with BSS; treated eye, detachment made with BSS, fasudil injected immediately afterward intravitreally to achieve a 10 mM concentration. SV2-labeled spots (white arrows) in the ONL indicate axonal retraction. (B) Binary images created from the green channel from (A). SV2-labeled spots indicated with red arrows. (C) Comparison of the average of attached and detached areas between BSS control and fasudil-treated eye. The axonal retraction was significantly reduced by 28.7% in fasudil-treated eyes compared to BSS (*P < 0.05, n = 5 animals, 500 images, ± SD).