Figure 6.

Rod cell degeneration in the detached retina after a 4-hour detachment. Fasudil (10 mM) was injected intravitreally 2 hours after the detachment was created; samples were collected an additional 2 hours later. Nuclei were stained with PI (red); synaptic terminals were labeled for SV2 (green). (A, B) Representative sections of retina from untreated (BSS) and treated (Fasudil) eyes. Densely stained (pyknotic) nuclei are present in the ONL of the untreated detached retina (arrowheads) where rod cell bodies reside. (C) Treatment with fasudil significantly reduced the number of densely stained nuclei in the ONL by approximately 55.7% compared to controls (BSS, 14.1 ± 3.5; fasudil, 6.3 ± 3.1 nuclei/mm ONL; ***P < 0.001, n = 4 animals, 400 images, ± SD).