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. 2017 May 29;16(1):755–763. doi: 10.3892/mmr.2017.6640

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — miR-146a regulates SGC-7901 cell apoptosis and proliferation. SGC-7901 cells were transfected with an miR-146a mimic or inhibitor, or scrambled controls, at a final concentration of 10 nM for 48 h. Reverse transcription-quantitative polymerase chain reaction confirmed (A) upregulation of miR-146a levels in mimic-transfected cells and (B) downregulation of miR-146a levels in inhibitor-transfected cells, compared with controls. Apoptosis detection by Annexin V/PI staining followed by flow cytometry in (C) miR-146a overexpressing and (D) miR-146a inhibited cells. (E) The percentage of Annexin V⁺ cells (Annexin V⁺/PI⁻ and Annexin V⁺/PI⁺ cells). (F) Caspase-3 activity was measured with the Caspase-3 Activity assay kit. Data are expressed as the mean ± standard deviation of six (E), four (A and B) or two (F) independent experiments. *P<0.05; **P<0.01. miR, microRNA; PI, propidium iodide; Ctrl, control.