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. 2017 May 29;16(1):755–763. doi: 10.3892/mmr.2017.6640

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — TAK1 regulates gastric cancer cell apoptosis. SGC-7901 cells were transfected with TAK1 siRNA or scrambled control at a final concentration of 20 nM, or TAK1-Flag plasmid, for 48 h. (A) Reverse transcription-quantitative polymerase chain reaction confirmed decreased TAK1 mRNA expression levels in si TAK1-transfected cells and (B and C) western blotting confirmed increased TAK1 protein expression levels in TAK1-Flag-transfected cells, compared with controls. Apoptosis detection by Annexin V/PI staining followed by flow cytometry in (D) TAK1 knockdown and (E) TAK1 overexpressing cells. The percentage of Annexin V⁺ cells (Annexin V⁺/PI⁻ and Annexin V⁺/PI⁺ cells) in (F) TAK1 knockdown and (G) TAK1 overexpressing cells. Data are expressed as the mean ± standard deviation and represent six independent experiments. *P<0.05; **P<0.01. TAK1, transforming growth factor β-activated kinase 1; si, small interfering; PI, propidium iodide; Ctrl, control.