Table I.
Gene (Homo sapiens) | Sequence (5′-3′) |
---|---|
miR-146a mimic | UGAGAACUGAAUUCCAUGGGUU |
Mimic NC | UUCUCCGAACGUGUCACGUTT |
miR-146a inhibitor | AACCCAUGGAAUUCAGUUCUCA |
Inhibitor NC | ACGUGACACGUUCGGAGAATT |
si TAK1 | AAAGCGTTTATTGTAGAGCTT |
si TAK1 NC | ACGUGACACGUUCGGAGAATT |
miR-146a-forward | CAGTGCGTGTCGTGGAGT |
miR-146a-reverse | GGGTGAGAACTGAATTCCA |
U6-forward | CTCGCTTCGGCAGCACA |
U6-reverse | AACGCTTCACGAATTTGCGT |
TAK1-forward | ATCAGCAGAGTAGCTGCGGT |
TAK1-reverse | GAGGAGCTTGCTGCAGAGT |
GAPDH-forward | AGGGCTGCTTTTAACTCTGGT |
GAPDH-reverse | CCCCACTTGATTTTGGAGGGA |
miR, microRNA; NC, negative control; TAK1, transforming growth factor β-activated kinase 1; si, small interfering.