Figure 4.

The inhibition of NOTCH2 attenuated the intracellular ROS and cell apoptosis induced by UVB. (A) The mRNA relative expression level of NOTCH2 in RPE cells with NOTCH2 inhibited radiated by UVB were determined by RT-qPCR. (B and C) Intracellular ROS and cell apoptosis were determined by flow cytometry in RPE cells with NOTCH2 inhibited by UVB. (D and E) The efficiency of NOTCH1 knockdown was determined by RT-qPCR and western blot analysis. (F and G) Cell apoptosis and intracellular ROS were determined by flow cytometry. The data are expressed as the mean ± standard deviation of three independent experiments. Statistical analysis was performed using Student's t-test. ^##P<0.01 vs. control without UVB radiation. **P<0.01 vs. control radiated by UVB without NOTCH2 inhibition. UVB, ultraviolet B; RPE, retinal pigment epithelium; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.