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. Author manuscript; available in PMC: 2017 Jun 23.
Published in final edited form as: Eur Cell Mater. 2016 Nov 18;32:257–270. doi: 10.22203/eCM.v032a17

Fig. 4.

Fig. 4

Mineralised deposits and expression of COL10 and OPN after 4 d AGE exposure indicate a direct relationship of AGEs on endochondral ossification in bovine cell culture. (a) Mineralised deposits were visible after 4 d AGE exposure in NP (top) and CEP (bottom). Inserts show higher magnification of representative regions. Arrows mark ectopic calcifications. (b) Immunocytochemistry with RAGE (top), COL10 (middle) and OPN (bottom) after 4 d culture. White arrows mark positive stained cells. (c) Quantification of RAGE, COL10 and OPN positive cells after 4 d culture; * p < 0.05; n = 4, COL10 and OPN; n = 4, RAGE.