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. Author manuscript; available in PMC: 2017 Jun 23.
Published in final edited form as: Nat Neurosci. 2016 Nov 7;19(12):1599–1609. doi: 10.1038/nn.4421

Figure 5. Involvement of the CXCL1 and CXCR2 in development of Type-B EAE and Type-A EAE with MHV68 infection.

Figure 5

(a) Transwell migration assay towards rCXCL1 using splenic CD4+ T cells harvested from indicated mice. n=4. (b) Levels of CXCL1 in serum from naïve mice or mice with Type-A or Type-B EAE mice on 9-dpi determined by ELISA. Naïve (n=5), Type-A (n=3), Type-B (n=5). P(Naive vs Type-A)=0.8676, t(6)=0.174, P(Naive vs Type-B)=0.0059, t(8)=3.722, P(Type-A vs Type-B)=0.0334, t(6)=2.749. (c) CXCL1 staining in the choroid plexus on 9-dpi. Scale bars, 20 μm. (d, e) Levels of CXCL1 in serum from Type-B EAE with or without LTβR-Fc treatment on 9-dpi. n=4. P=0.0174, t(6)=3.252. Scale bars, 20 μm. (f) Score of Type-A or Type-B EAE treated with or without SB225002. Vehicle-treated group (n=5), SB225002-treated group (n=7). P=0.002, t(10)=5.634. (g) EAE score in Lck-Cre Cxcr2fl/fl mice (CXCR2-cKO) and Cxcr2fl/fl mice (control). *; p<0.05. (n=8). P=0.0192, t(14)=2.646. (h) Representative LFB-stained images with red lines indicating regions of demyelination at 17-dpi. Scale bars, 200 μm. (i) Percentages LTα+ DCs in DLNs and CXCR2+CD4+T cells in spleens of mice at 9-dpi immunized with a Type-A EAE induction method with or without MHV68 infection. (n=4). P=0.0002, t(6)=7.72, P=0.0026, t(6)=4.931. (j) Serum levels of IL-1β and IFNβ at 9-dpi in naïve mice and mice immunized with a Type-A EAE induction method with or without MHV68 infection. n=4. IL-1β: P(Naive vs Type-A)=0.0152, t(6)=3.361, P(Naive vs Type-A+MHV68)=0.3925, t(6)=0.9212, P(Type-A vs Type-A+MHV68)=0.0259, t(6)=2.942. CXCL1: P(Naive vs Type-A)=0.8498, t(6)=0.1978, P(Naive vs Type-A+MHV68)=0.0174, t(6)=3.252, P(Type-A vs Type-A+MHV68) 0.0205, t(6)=3.124. (k) EAE scores of mice Type-A-induced mice with MHV68 infection. Treatment was with either LTβR-Fc or SB225002. Negative control groups did not receive the treatments (n=4). All statistical analyses in this figure were performed by two-tailed unpaired Student’s t-test. All the experimental data and images are representatives from at least 2 similar experiments for each.