Figure 3.

Cell suspension model confirmed cell-associated ApoE accumulation. (A) Suspended RPE cells from a 51-year-old donor with ApoE phenotype E3/E3 and CFH_HH402 variant were incubated with either normal sheep IgG (1.2 mg/mL) or S58 (1.2 mg/mL) for 30 minutes and then treated with 6% C1q-Dep for 4.5 hours at 37°C in a shaking incubator followed by cell lysis and SDS-PAGE analysis of total proteins (30 μg). (B) Quantitation of cell-associated ApoE relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) shown in (A) was determined by densitometry. *P = 0.02 versus sheep IgG+C1q-Dep. Data are representative of two separate experiments in two donors with similar results.