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. 2017 Jul;58(7):3073–3085. doi: 10.1167/iovs.16-20083

Figure 6.

Figure 6

Detection of cleaved recombinant ApoE human protein in serum-treated cells. RPE cells from a 61-year-old donor with ApoE phenotype E3/E4 and CFHYH402 variant were primed with or without S58 (1.2 mg/mL) for 30 minutes and then treated with 6% either C1q-Dep or HiC1q-Dep for 5 hours with or without recombinant ApoE human protein with N-TRX.His tags (4 μg/mL). Total proteins (15 μg) were separated by SDS-PAGE. Data are representative of four separate experiments in two donors with similar results. *S58 IgG heavy chain.