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. 2017 Jul;58(7):3073–3085. doi: 10.1167/iovs.16-20083

Figure 8.

Figure 8

CHX partially inhibited complement-mediated ApoE accumulation. RPE cells from a 51-year-old donor with ApoE phenotype E3/E3 and CFHHH402 variant (A) and a 61-year-old donor with ApoE phenotype E3/E4 and CFHYH402 variant (B) were preincubated with CHX at 20 μg/mL for 1.5 hours, primed with or without S58 (1.2 mg/mL) for 30 minutes, and then treated with either 6% C1q-Dep or HiC1q-Dep for 5 hours. CHX was continuously present when cells were primed with S58 and incubated with complement serum. Total proteins (15 μg) were separated by SDS-PAGE for Western blot. The quantity of ApoE relative to GAPDH shown in (A, B) was determined by densitometry (labeling 1–5 represents five treatment groups). *P < 0.05 vs. C1q-Dep. **P < 0.05 vs. S58+C1q-Dep. Data are representative of six separate experiments in three donors with similar results.