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Cells were inoculated in 24 well plate (non-treated tissue culture polystyrene) at the concentration of 5×103/mL (upper) or 1×104/mL (lower). Odontogenic reagents were added in the same manner as Figure 6, and staining was conducted at 10 days of culture. Calcification nodules were clearly observed in Fn(10-50) and PCOL300 in both low and high cell seeding density
