Figure 1.

An acetate-driven TCA cycle in diverse bacteria. (A) The TCA cycle (green) and the asct modification (pink). (B) Restoration of TCA cycle function by asct from diverse bacteria (labeled 1–6) in E. coli succinyl-CoA synthetase (SCS) knockouts. Operon structure of sucCD in E. coli is shown at upper left (not to scale); black arrows indicate promoters. Δ, deletion; ::kan, kanamycin resistance gene insertion. Control strains, carrying a plasmid without asct (pBad-EBFP2), are denoted by dashed lines. Colour of lines indicate E. coli background of asct-complemented or control strains; strains are listed in Supplementary Table 1. Growth curves represent average of three replicates; see Supplementary Table 2 for means and standard deviations at each time point. (C) Phylogeny of asct from representative species of phyla Bacteroidetes, Actinobacteria, and Proteobacteria (α, β, γ; classes Alpha-, Beta-, and Gamma-proteobacteria, respectively). Circles indicate nodes with > 95% bootstrap support from maximum-likelihood analysis. Taxa used to build this tree are shown in Supplementary Fig. 2. Numbered labels indicate positions of taxa in (B), with color scheme reflecting presence (blue) or absence (red) of co-occurring sucCD.