Fig. 2.

Representative quantitative phase images of three different confluence levels: (a) low confluence (LC), with fewer than 20 neurons per field of view; (b) medium confluence (MC), with 30 to 50 neurons per field of view; (c) high confluence (HC), with more than 50 neurons per field of view. (d) Colorbar indicates phase shift in radians; (e–g) sample images of neurites traced by NeuronJ. Neurites are labeled as follows: axon, red; secondary, yellow; tertiary, cyan; dendrite, blue; type 06, green; and default, pink.