Figure 2.

Detection of P. falciparum associated with HUVECs by Giemsa staining. P. falciparum iRBCs or uninfected RBCs were added to HUVEC cell cultures at the indicated times, as described in Materials and Methods. A. Following the HUVEC association assay, cells were stained with Giemsa. HUVECS incubated with P. falciparum iRBCS (top row) show multiple parasite-like bodies (arrow). No such bodies are seen in HUVECs incubated with uninfected RBCs (bottom row). B. The percentage of HUVEC cells containing 0, 1, or > 1 parasite-like bodies was calculated for each time point.