Figure 6.

Measurement of P. falciparum rRNA and DNA associated with HUVECs by SYBR Green quantitative PCR. P. falciparum infected RBCs were added to HUVEC cell cultures for the indicated times, as described in Materials and Methods. Following these incubations, RNA and genomic DNA were purified from HUVECS. A. P. falciparum SSU rRNA amounts from HUVECs as measured by reverse-transcription qPCR. No PCR products were detected in RNA samples not treated with reverse-transcriptase (data not shown). Error bars represent standard deviation from three independent replicates. B. The SSU rRNA gene was amplified from P. falciparum genomic DNA (gDNA) from HUVECs and measured by qPCR. P. falciparum RNA and DNA amounts were normalized to human GAPDH RNA and DNA amounts, respectively. As a control, HUVECs were incubated with uninfected RBCs. No P. falciparum-specific PCR products were detected (data not shown). All experiments were done in triplicate.