Fig. 3.
Characterisation of ligand binding in the near-UV region (260–230 nm). Difference spectrum of (A) VanSA in the presence of 5-fold molar equivalent of compounds (vancomycin, teicoplanin, N-acetylmuramic acid, D-Ala-D-Ala and Ala-D-y-Glu-Lys-D-Ala-D-Ala); (B) titration of vancomycin and (C) titration of teicoplanin. Binding constant (kd) calculated from change in CD (mdeg) at (B) 286 nm and (C) 292 nm, plotting against respective concentration (M) of GPA and fitting with a (B) Hill1 or (C) BidoseResponse function. Standard deviation (n = 4) shown by error bars.