Figure 2.
CSF1R inhibition eliminates microglia in brain. (a) Schematics of microglia depletion and experimental design. Mice were treated with 40 mg/kg body weight PLX3397 for 21 days before ICH induction and continued until the end of experiments. (b,c) After PLX3397 treatment for 21 days, ICH was induced by injection of 0.0375U collagenase. After ICH, treatment was continued until mice were sacrificed. At day 3 after ICH, the number of brain microglia (CD45intCD11b+) was analyzed by flow cytometry in the following groups of mice: ICH and non-ICH control mice receiving PLX3397 or vehicle. Gating strategy (b) and summarized results from two independent experiments (c) are shown. Mean ± s.e.m. n = 6 mice per group, **p < 0.01.