Figure 3.

CSF1R inhibition attenuates neurodeficits and brain edema in two mouse models of ICH. (a–f) PLX3397 reduced neurodeficits, lesion volume, and perihematomal edema volume after ICH. Representative 7T MRI images and quantification of lesion volume and perihematomal edema volume in ICH mice receiving PLX3397 treatment versus untreated controls. ICH was induced by injection of autologous blood (a) or collagenase (b), mice treated with PLX3397 had reduced neurodeficits than untreated controls at indicated time points after ICH induced by injection of autologous blood (a) and collagenase (b). Multi-modal 7T MRI were performed to visualize lesion (T2) and hematoma (SWI). Perihematomal edema volume was calculated by subtracting the hematoma volume from lesion volume. Red lines delineate lesion area, yellow shaded regions represent hematoma area. The method used to determine perihematomal edema volume is depicted in Supplemental Fig. 1A. At day 3 after ICH, lesion volume and brain edema were assessed by this method in autologous blood model (c) and collagenase model (d). At day 3 after ICH, PLX3397 treatment decreased brain water content in ipsilateral hemisphere in autologous blood model (e) and collagenase model (f). Mean ± s.e.m. n = 10 mice per group from two independent experiments, *p < 0.05.