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. Author manuscript; available in PMC: 2017 Jun 23.
Published in final edited form as: Int Immunopharmacol. 2015 Aug 24;28(2):1102–1105. doi: 10.1016/j.intimp.2015.07.039

Figure 2.

Figure 2

Anti-fibrotic effects of single or multiple low dose E4 in bleomycin-induced pulmonary fibrosis in vivo. Bleomycin (BLM) was intratracheally administrated to male C57BL/6 mice in combination with oral E4. Lungs were harvested 21 days post-treatment. The amount of lung collagen was quantified using hydroxyproline assay. Total collagen was expressed as μg collagen per lung. A. Lungs were harvested from mice treated with PBS (N=14), BLM (N=14), BLM and E4 20μg (N=9), BLM and E4 10μg (N=6), BLM and E4 5μg (N=6), and BLM and E4 1μg (N=6). B. E4 was administrated 0, 3, and 6 days after BLM treatment. Lungs were harvested from mice treated with PBS (N=8), BLM (N=8), BLM and E4 10μg x3 (N=4), and BLM and E4 5μg x3 (N=7).