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. 2017 Jun 23;12(6):e0179614. doi: 10.1371/journal.pone.0179614

Fig 1. Lipo-PGE1 markedly reduces collagen expression and production in HDFs.

Fig 1

(A) HDFs were harvested after Lipo-PGE1 treatment (2.5–40 ng/mL) for 24 h. Total RNA was extracted and cDNA was synthesized for real-time RT-PCR. Bars indicate mean ± SD of three independent experiments, each with triplicate samples. *P<0.05 (B) Effects of Lipo-PGE1 on expression of type I collagen protein in HDFs. Cells were harvested after 36 h and collagen expression was analysed by Western blot using anti-type I collagen antibody. (C) HDFs were harvested after Lipo-PGE1 treatment (5 ng/mL) for the indicated duration. Total collagen was detected by the Sircol assay, which was performed in triplicate. Data are mean ± SD. *P<0.01 vs. control at the indicated time point.