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(A) Organization of the expression cassette for L-NHase. nhlBAE, wild-type L-NHase gene; nhlBrbsAE, the intervening sequences shortened gene with one enhanced RBS ahead of nhlA; nhlBrbsArbsE, the intervening sequences shortened gene with two enhanced RBSes ahead of nhlA and nhlE. (B) Gene organization of the constructed genes used for H-NHase expression.
