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. 2017 Jun 23;12(6):e0179833. doi: 10.1371/journal.pone.0179833

Fig 5. Production of NHase in recombinant E. coli and in native R. rhodochrous J1.

Fig 5

Cell growth and NHase activity of (A) the genetically engineered E. coli for H-NHase, (B) L-NHase, and (C) R. rhodochrous J1. (D) Comparison of nicotinamide-tolerance of NHases from the cells of R. rhodochrous J1 (column 1) and the H-NHase, and L-NHase from the genetically engineered E. coli (column 2 and 3). The reduction ratio (the proportion of the reduced 3-cyanopyridine amount in the reaction with and without 0.5 M nicotinamide) was calculated.