Table 1. Primers used for NHase expression.

Name	Sequence(5’-3’)	Length (bp)
B-up	AAGGAGATATACATATGGATGGAATCCAC	29
E-down	CCCAAGCTTCTACCCGTCGGAGTCAGTGGTGC	32
1RBS-up	CGAACCGTATCTGCTACCGGCCTGAAAGGAGATATAGATATGACCGCCCACAATCCCG	58
1RBS-down	CGGGATTGTGGGCGGTCATATCTATATCTCCTTTCAGGCCGGTAGCAGATACGGTTCG	58
2RBS-up	CACCACACCCAGCAAGGCCTGAAAGGAGATATAGATATGCCCCGACTCAACGAACAA	57
2RBS-down	TTGTTCGTTGAGTCGGGGCATATCTATATCTCCTTTCAGGCCTTGCTGGGTGTGGTG	57
Strep -B-up	GTTTAACTTTAAGAAGGAGATATAGATATGGCAAGCTGGAGCCACCCGCAGTTCGAAAAGGATGGAATCCACGACCTCGGTGGCC	85
Strep -B-down	GGCCACCGAGGTCGTGGATTCCATCCTTTTCGAACTGCGGGTGGCTCCAGCTTGCCATATGTATATCTCCTTCTTAAAGTTAAAC	85

The underlined sequences are the restriction sites. The bold and underlined sequences are the ribosome binding sites and spacer sequences.