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. 2017 Jun 23;12(6):e0180257. doi: 10.1371/journal.pone.0180257

Fig 3. Immunoprecipitation of differently tagged OATP1B3 proteins.

Fig 3

Membrane fractions isolated from HEK293 cells transiently transfected with OATP1B3-His and OATP1B3-FLAG (A) or OATP1B3-HA and OATP1B3-FLAG (B) and treated with or without DTSSP were solubilized. Immunoprecipitation was performed with Anti-His or Anti-FLAG antibodies (A), or Anti-HA or Anti-FLAG antibodies (B). The eluted proteins were detected with Anti-His antibody (A) or Anti-HA antibody (B).