Fig. 4.

Western blot analysis of VPS34 levels in THP-1-derived macrophages. a UVRAG-linked VPS34 was detected by co-IP. The results are shown as the relative UVRAG-linked VPS34 normalized to total VPS34. b Phosphorylated AMPK was detected by western blot. Data are shown as the relative p-AMPK levels normalized to total AMPK. (1) NT, THP-1-derived macrophages were maintained in RPMI1640 culture medium for 6 days; (2) M.tb, M.tb-infected macrophages were maintained in RPMI1640 culture medium for 6 days; (3) M.tb+SD(CTL), M.tb-infected macrophages were maintained in RPMI1640 culture medium containing DMSO(the solution medium of SD) for 6 days. DMSO was added to the culture medium at the same volume as SD; (4) M.tb+SD, M.tb-infected macrophages were maintained in RPMI1640 culture medium containing 6 μM SD for 6 days; (5) M.tb+SD+DOR(CTL), M.tb-infected macrophages were maintained in RPMI1640 culture medium containing 6 μM SD and the solution medium of DOR(DMSO) for 6 days. The solute medium of DOR was added to the culture medium based on the volume of DOR; (6) M.tb+SD+DOR, M.tb-infected macrophages were maintained in RPMI1640 culture medium containing 6 μM SD and 1 μM DOR for 6 days. Data are shown as the mean ± S.D. of 4 independent experiments carried out in triplicate. *P < 0.05 versus NT group; ^※P < 0.05 versus M.tb group; ^★P < 0.05 versus M.tb+SD group