Figure 2. The effect of thalidomide on mouse platelets in vitro.

Isolated mouse platelets (2.5 × 10⁸/ml) were treated with indicated concentrations of thalidomide followed by measuring (A) expression of P-selectin, (B) fibrinogen (Fng) binding, or (C) expression of α_IIb by flow cytometry. Platelet aggregation in mouse washed platelets after thalidomide treatment in response to threshold concentration of (D) collagen (1 μg/ml) or (E) ADP (10 μM) was performed in the presence of fibrinogen (0.5 mg/ml). CRP (10 μg/ml) was used as a positive control (“+”) for mouse platelet activation. All the data were acquired from at least three independent experiments.