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. 2017 Mar 15;8(22):35835–35847. doi: 10.18632/oncotarget.16214

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Copyright: © 2017 Khandia et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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(A) SDS-PAGE analysis of E. coli expressed 6X His Tagged PA Protein (B) SDS-PAGE analysis of E. coli expressed 6X His Tagged LF Protein (Lane 1 corresponds to Rosettablue(DE3)pLysS E.coli cell lysate; lane 2- Total cell pellet of induced culture; Lane 3-Soluble fraction of cell lysate; Lane 4-Inclusion body fraction of cell lysate; Lane 5-Ni-NTA purified PA/ LF protein; Lane M- Molecular weight markers) (C) Western blot analysis of purified PA and LF proteins (~63kDa PA protein and ~85kDa LF protein) (Lane 1 corresponds to Purified PA63 protein; lane 2-Purified LF protein; Lane M- Molecular weight markers).