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. 2017 Mar 16;8(22):35890–35901. doi: 10.18632/oncotarget.16271

Figure 4. Effect of BMP9 on the expression of leptin and ObR in the co-culture system.

Figure 4

(A) The ObR expression in MDA-MB-231 cells was detected by immunofluorescence staining (×400). (B) The effect of BMP9 on the expression of leptin and ObR in MDA-MB-231 cells was detected by Western blotting. (C) The effect of BMP9 on the expression of leptin in pre-adipocytes or adipocytes was evaluated by Western blotting. All experiments were performed in triplicate. BMP9 had no effect on the expression of leptin and Ob-R of MDA-MB-231 cells, but inhibited the expression of leptin in the pre-adipocytes and adipocytes. Co-pre-Blank: MDA-MB-231 + pre-adipocytes; Co-pre-GFP: MDA-MB-231/AdGFP + pre-adipocytes; Co-pre-BMP9: MDA-MB-231/AdBMP9 + pre-adipocytes; Co-adi-Blank: MDA-MB-231 + adipocytes; Co-adi-GFP: MDA-MB-231/AdGFP + adipocytes; Co-adi-BMP9: MDA-MB-231/AdBMP9 + adipocytes.