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. 2017 Mar 21;8(22):35946–35961. doi: 10.18632/oncotarget.16412

Figure 4. Reparixin inhibits stemness features of TC cells.

Figure 4

(A–B) Effect of Reparixin (30 μM) on mRNA levels of the basal and IL-8 induced-stemness markers (OCT3/4, NANOG, ABCG2, ALDH, SOX 2) was evaluated by real time PCR in 8505c, CAL62 and SW1736 cells. Normalization to β-actin mRNA levels was used. For each target (x-axis), the expression levels were calculated relative to the expression levels in untreated cells, arbitrarily considered equal to 1 (dotted line). Experiments were performed in triplicate. *p < 0.05 compared to untreated cells (dotted line); §p < 0.05 compared to IL-8 treated cells. (C) Cytofluorimetric evaluation of SOX 2 stemness marker levels in Reparixin (30 μM)-treated CAL62 and SW1736 cells in the presence or absence of IL-8 (100 ng/ml). *p < 0.05 compared to untreated cells (NT); §p < 0.05 compared to IL-8 treated cells. (D) Percent of Aldefluorhigh cells in Reparixin (30 μM)-treated 8505c, CAL62 and SW1736 cells, in the presence or absence of IL-8 (100 ng/ml). *p < 0.05 compared to untreated cells (NT); §p < 0.05 compared to IL-8 treated cells.