Figure 2. Up-regulation of P2Y₂R expression in idiopathic pulmonary fibrosis.

(A) Expression of P2Y₂ receptor subtypes on BAL cells derived from IPF patients (n = 16) and healthy individuals (n = 10) was determined by quantitative RT-PCR. (B) Neutrophils were isolated from whole blood of IPF patients (n = 9) and healthy individuals (n = 9) and P2Y₂R expression was measured by quantitative RT-PCR. (C) Neutrophils were isolated from whole blood derived from IPF patients (n = 5) or healthy volunteers (n = 6). Migration in response to ATP or fMLP as a positive control was assessed using the Boyden chamber. *p < 0.05; ***p < 0.001.