Figure 5. P2Y₂R-deficient animals are partially protected from bleomycin induced lung injury and fibrosis.

P2Y₂R-deficient or wild type mice received an i. t. injection of BLM or vehicle on day 0 and were killed 7, 14 or 21 days later. (A) Total and differential cell counts of the BALF were performed. (B) BALF cytokines were measured by ELISA. (C) Collagen contents were quantified in the BAL fluid by Sircol assay (left) and on histological lung slides (middle). α1 procollagen expression was determined by quantitative real time PCR (right). (D) Histological staining of lung slides (H&E). (n = 4–14 per group). *p < 0.05 compared to BLM-treated wt animals at the same time point.