Figure 3. RT-PCR validation of 4 DEGs specific for poorly differentiated HCC.

GAPDH mRNA was used as an internal control. Expression data were obtained as 2^−ΔΔCT relative to adjacent non-tumor tissue values. N_PD and T_PD represented matched adjacent non-tumor tissue specimens (n = 15) and poorly differentiated HCC tissue specimens (n = 15) respectively. N_MD and T_MD represented matched adjacent non-tumor tissue specimens (n = 18) and moderately differentiated HCC tissue specimens (n = 18) respectively. N_WD and T_WD represented matched adjacent non-tumor tissue specimens (n = 14) and well differentiated HCC tissue specimens (n=14) respectively. (A) NOVA1; (B) NSMCE2; (C) KIAA0196; (D) AQP9. ^#P < 0.05 vs T_MD and T_WD.